Determination of lamotrigine in human serum by liquid chromatography

摘要

A rapid high-performance liquid chromatographic method was developed using a short silica column (30 mm×4.6 mm) with an aqueous methanol mobile phase consisting of methanol-water-NH_4H_2PO_4 (94:5.96:0.04) adjusted to final apparent pH of 5.0 and pumped at a flow-rate of 1 ml/min. Ultraviolet detection was carried out at a wavelength of 280 nm, and serum samples were prepared of HPLC analysis by extraction into dichloromethane after basificatio. Lamotrigine was eluted at 0.96 min. Within-day variation of the method was 4.64% at 0.75 #mu#g/ml and 2.37% at 6.0 #mu#g/ml, and day-to-day variation was 9.10% at 0.75 #mu#g/ml and 7.28% at 6.0 #mu#g/ml.