Simultaneous high-performance liquid chromatographic determination of urinary mandelic and phenylglyoxylic acids as indirect evaluation of styrene exposure

摘要

Styrene is rapidly metabolised to mandelic acid (MA) and phenylglyoxylic aicd (PGA), which are excreted in urine. In this work, we ahve developed a simple, sensitive and specific high-performance liquid chromatographic method with minor sample preparation procedures for hte simultaneous detemriantion of MA and PGA in urine of workers expose dto styrene. Moreover, urine samples from workers of two plastic factories were analysed, styrene exposure levels of the workers were estimated and data obtained from the two factories were compared.