Determination of acrolein by headspace solid-phase microextraction gas chromatography and mass spectrometry

摘要

We developed a heads pace solid-phase microextraction (headspace SPME)method to measure acrolein in human urine. This new technique resolves some problems with the headspace gas chromatography and mass spectrometry (GC-MS) method which we developed previously. With the original method, a column and a filament were damaged by the injection of air. A 0.5-ml urine (or phosphate-buffered saline) sample in a glass vial containing propionaldehyde as an internal standard was heated for 5 min. The SPME fiber (65 #mu#m carbonwax-divinylbenzene fiber) was exposed to the headspace and then Inserted into a GC-MS instrument in which a DB-WAX capillary column (30 m * 0.32 mm, film thickness 0.5 #mu#m) was installed. The total analysis time was 15 min. The inter-assay and intra-assay coefficients of variation were 10.07 and 5..79%, respectively. The calibration curve demonstrated good linearity throughout concentrations ranging from 1 to 10 000 nM .The headspace SPME method exhibits high sensitivity and requires a short analysis time as well as the previous method. We conclude that this method is useful to measure urinary acrolein.