iPSC-derived human cardiac progenitor cells improve ventricular remodelling via angiogenesis and interstitial networking of infarcted myocardium

摘要

We investigate the effects of myocardial transplantation of human induced pluripotent stem cell (iPSC)-derived progenitors and cardiomyocytes into acutely infarcted myocardium in severe combined immune deficiency mice. A total of 2 x 10(5) progenitors, cardiomyocytes or cell-free saline were injected into peri-infarcted anterior free wall. Sham-operated animals received no injection. Myocardial function was assessed at 2-week and 4-week post-infarction by using echocardiography and pressure-volume catheterization. Early myocardial remodelling was observed at 2-week with echocardiography derived stroke volume (SV) in saline (20.45 +/- 7.36 l, P < 0.05) and cardiomyocyte (19.52 +/- 3.97 l, P < 0.05) groups, but not in progenitor group (25.65 +/- 3.61 l), significantly deteriorated as compared to sham control group (28.41 +/- 4.41 l). Consistently, pressure-volume haemodynamic measurements showed worsening chamber dilation in saline (EDV: 23.24 +/- 5.01 l, P < 0.05; ESV: 17.08 +/- 5.82 l, P < 0.05) and cardiomyocyte (EDV: 26.45 +/- 5.69 l, P < 0.05; ESV: 18.03 +/- 6.58 l, P < 0.05) groups by 4-week post-infarction as compared to control (EDV: 15.26 +/- 2.96 l; ESV: 8.41 +/- 2.94 l). In contrast, cardiac progenitors (EDV: 20.09 +/- 7.76 l; ESV: 13.98 +/- 6.74 l) persistently protected chamber geometry against negative cardiac remodelling. Similarly, as compared to sham control (54.64 +/- 11.37%), LV ejection fraction was preserved in progenitor group from 2-(38.68 +/- 7.34%) to 4-week (39.56 +/- 13.26%) while cardiomyocyte (36.52 +/- 11.39%, P < 0.05) and saline (35.34 +/- 11.86%, P < 0.05) groups deteriorated early at 2-week. Improvements of myocardial function in the progenitor group corresponded to increased vascularization (16.12 +/- 1.49/mm(2) to 25.48 +/- 2.08/mm(2) myocardial tissue, P < 0.05) and coincided with augmented networking of cardiac telocytes in the interstitial space of infarcted zone.