Development of a DNA Interaction Test with Small Molecules Still Grafted on Solid Phase

摘要

Gene therapy, as some other approaches for selective delivery of medicines, uses a carrier in order to encapsulate therapeutical DNA and ensure its travel to the desired treatment site. The usual molecular family used to do so is positively charged molecules, such as cationic lipidic or polymeric derivatives. Nevertheless, in the efficiency of such molecules for wrapping and carrying and performing transfection in vitro, cationic DNA vectors suffer from major faults, such as fast clearance in vivo; interactions with biomolecules in the serum, which may lead to strokes; and finally, some cellular toxicity. There is thus still a need for research in order to find new functions and molecules that can advantageously replace cationic vectors in these approaches