The Removal of the t-BOC Group by Electrochemically Generated Acid and Use of an Addressable Electrode Array for Peptide Synthesis

摘要

Rapid developments in the field of DNA arrays have led to a number of methods for their in situ synthetic preparation,including photolithography using both fixed and programmable masks,ink jet printing of reagents,and electrochemical deprotection techniques.These techniques have led to a number of commercially available DNA microarray products.The in situ synthetic preparation of a peptide array,however,has proven significantly more challenging.An in situ synthetic peptide array using photomasking techniques was originally reported by Fodor in 1991.This methodology was extended in 2000 to an addressable masking technique.For lower density arrays,synthesis using spotted reagents has also proven useful.The immobilization of biosynthe-sized proteins directed by DNA hybridization has been shown for arrays of up to 12 proteins.Given the synthetic challenges of constructing a peptide array,most recent work involving peptide arrays has utilized arrays produced by spotting presynthesized peptides or isolated proteins.