Structural models for the self-assembly and microtubule interactions of gamma-, delta- and epsilon-tubulin

摘要

alpha beta -tubulin heterodimers self-assemble to form microtubules nucleated by gamma -tubulin in the cell. gamma -tubulin is believed to recruit the alpha beta -tubulin dimers that form the minus ends of microtubules, but the molecular mechanism of this action remains a matter of heated controversy. Still less is known about the function and molecular interactions of delta -tubulin and epsilon -tubulin, delta -tubulin may seed the formation of the C triplet tubules in the basal bodies of Chlamydomonas and epsilon -tubulin is known to localize to the centrosome in a cell cycle-dependent manner, Using the structure of alpha beta tubulin as a model, we have analyzed the sequences of gamma-, delta- and epsilon -tubulin in regions corresponding to different polymerization interfaces in the tubulin alpha beta dimer, The sequence comparisons sometimes show clear conservation, pointing to similar types of contacts being functionally important for the new tubulin considered. Conversely, certain surfaces show marked differences that rule out equivalent interactions for non-microtubular tubulins, This sequence/structure analysis has led us to structural models of how these special tubulins may be involved in protein-protein contacts that affect microtubule self-assembly. delta -tubulin most likely interacts longitudinally with alpha -tubulin at the minus ends of microtubules, while epsilon -tubulin most likely binds to the plus end of beta -tubulin, Conservation of key residues in gamma -tubulin suggests that it is capable of longitudinal self-assembly. The implications for the protofilament and template models of nucleation are considered. [References: 36]