A Size Exclusion Chromatography Laboratory with Unknowns for Introductory Students

摘要

Size exclusion chromatography is an important technique in the separation of biological and polymeric samples by molecular weight. While a number of laboratory experiments have been published that use this technique for the purification of large molecules, this is the first report of an experiment that focuses on purifying an unknown small molecule from a protein. Students must then determine the structure of the unknown using spectral determination.