A P450 Metabolism Experiment for Undergraduate Biochemistry Laboratories

摘要

A laboratory experiment is described to provide students hands-on experience in learning some aspects of microsomal P450-catalyzed metabolism. Undergraduate students in the biochemistry laboratories detect and quantify the metabolites produced from butylated hydroxytoluene (BHT) by liver microsomes using gas chromatography-mass spectrometry (GC-MS) techniques. The laboratory provides training to students for handling active microsomes, sample cleanup of biological matrices, extraction of organic metabolites, GC-MS analysis, data interpretation of complex mixtures, and collaborative work.