Sulfation of Hydroxyethyl Cellulose by N(SO3Na)3 and the Anticoagulant Activity of Sulfated Hydroxyethyl Cellulose

摘要

Hydroxyethyl cellulose sulfate (HECS) was synthesized by sulfation of hydroxyethyl cellulose with N(SO3Na)(3), which was manufactured by reaction of sodium bisulfite and sodium nitrite. Barium sulfate nephelometry and light scattering method were used to determine degrees of substitution (DSs) and molecular weights (M(w)s), respectively. Sulfate products with DS values from 0.26 to 1.88 and M-w in the range of 12.1-54.8kDa were obtained at temperatures from 30 degrees C to 80 degrees C. Furthermore, the anticoagulant activity of HECS with different DSs, concentrations, and M(w)s was studied. Clotting assays revealed that the introduction of sulfate groups into hydroxyethyl cellulose could improve its anticoagulant activity.