The influence of chinese herbal compound on the chronic restraint stress rat cortex and hippocampus BDNF, TrkB

摘要

Objective: To probe into the changes of rat cortex and hippocampus BDNF, TrkB when they are under the chronic restraint stress and the influence of the Chinese herbal compound of Xiaoyaosan, Buzhong Yiqi pill and Guifu Dihuang pill on them. Method: The special restriction bracket was used to restrict them for 7-21 days with 3 hours every day in order to construct the rat restraint stress model. The immunohistochemistry method combined the image analysis was used to detect the influences of traditional Chinese medicine on the changes of rat cortex and BDNF in hippocampus CA1 and TrkB. Results: After the continuous restrain for 7 days and 21 days, the cerebral frontal cortex and the BDNF in hippocampus CA1 of the rats were significantly lower than the control group (P < 0.05, P < 0.01). The 21d model groups were more obvious. After the continuous restrain for 7 days and 21 days, the cerebral frontal cortex and the TrkB in hippocampus CA1 of the rats were significantly higher than the control group (P < 0.05, P < 0.01). All the 3 Chinese herbal compounds can increase the integral absorbance of the cortex BDNF and the positive cell number of BDNF in hippocampus. Xiaoyaosan can decrease the positive cell number of TrkB in hippocampus and cortex integral absorbance of hippocampus TrkB. Buzhong Yiqi Pill and Guifu Dihuang pill can decrease the integral absorbance of TrkB in cortex. Guifu Dihuang pill can decrease the positive cell number of the cortex TrkB. The effect of Xiaoyaosan on increasing the BDNF in cortex and hippocampus is more obvious than that of Buzhong Yiqi pill and Gufu Dihuang pill. Conclusion: The decrease of cortex and BDNF in cortex and hippocampus CA1 participated in the changes of the chronic stress. The Chinese herbal compound with the effects of soothing the liver, invigorating the spleen and tonifying the kidney has the reversal effect to some degree. But the effect of the Xiaoyaosan is stongest, better than Buzhong Yiqi pill and Guifu Dihuang pill.