An ImproYed Method to Knock Out the msd Gene of Salmonella emterlcm Serovar Pulloriim

摘要

An asd-deleted (AascZ) mutant of Salmonella enterica serovar Pullorum (SP) was constructed using an improved method of gene knockout by combining the 7r-suicide plasmid system with the Red Disruption system. The asd gene was efficiently knocked out by the recombinant suicide vector, which replaced the asd gene with the Cm~R gene. Based on the balanced lethal host-vector system, the phenotype of the Aasd mutant was further defined. The improved method was simpler and more effective than previously reported conventional methods.