Cell adhesion and proliferation studies on semi-interpenetrating polymeric networks fsems-IPNs) of polyacrylamide and gelatin

摘要

In this study, the effect of feed composition, degree of hydrophilicity, and internal morphology has been investigated for cell proliferation potential of the polyacrylamide/gel-atin (PAm/G) semi-interpenetrating polymeric network (semi-IPNs). Polycaprolactone diacrylate was used to cross-link polyacrylamide chains. Scanning electron microscopy (SEM) micrographs demonstrate uniformly distributed porous structure with internal diameter in the range of 75-175 mum, dependent on matrix compositions. Water-air contact angle was found in the range of 49deg +- 0.22 to 89deg +- 0.14 (p < 0.02) suggesting varying degree of hydrophilicity of the hydrogel surface. In addition, protein adsorption study showed 45 +- 0.14 mug to 64 +- 0.12 mug (p < 0.01) of protein adsorbed per cm2 of hydrogel. Quantitative estimation of cell adhesion and proliferation was carried out by DNA quantification using fluorimetric assay method (p < 0.02). Microscopic images of proliferative cells on semi-IPNs by fluorescent and inverted phase contrast supported the findings of DNA quantification. Contact angle in the range of 63-69deg in association with 52-59 ng/cm2 protein absorption and 115-150 nm pore size was found optimum for fibroblast proliferation on PAm/G semi-IPN scaffolds. The newly developed semi-interpenetrating network may serve as a potential scaffold for soft tissue-engineering applications.