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Efficient chimeric promoters derived from full-length and sub-genomic transcript promoters of Figwort mosaic virus (FMV)

机译:源自嵌合花叶病毒(FMV)的全长和亚基因组转录本启动子的高效嵌合启动子

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摘要

Addition of multiple repeats of the FS3 upstream activation sequence (FS3-UAS, -270 to -60) intra-molecularly to the TATA containing core-domain of the FS3 (-151 to +31) promoter resulted in 2-3-folds enhanced promoter activity. The chimeric promoter, FS3-UAS-3X with maximum activity, showed 3.31 times stronger activity in root vascular tissue compared to FS3 promoter and could be used efficiently in translational research
机译:分子内将FS3上游激活序列(FS3-UAS,-270至-60)的多个重复添加到含有FS3(-151至+31)启动子核心结构域的TATA中,导致增强了2-3倍启动子活性。嵌合启动子FS3-UAS-3X具有最大活性,与FS3启动子相比,在根血管组织中的活性高3.31倍,可以有效地用于翻译研究

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