Dynamic Seeding and Perfusion Culture of Hepatocytes with Galactosylated Vegetable Sponge in Packed-Bed Bioreactor

摘要

A galactose moiety was introduced into the fiber surface of a vegetable sponge by the covalent binding of lactobionic acid.The galactosylated sponge was used as scaffold for the culture of rat hepatocytes in a packed-bed bioreactor.Hepatocytes could be dynamically seeded into and uniformly distributed throughout the scaffold,and the immobilized cells maintained high albumin and urea production rates during long-term perfusion culture.The hepatocytes showed an increasing albumin production rate from 49 to 109 mu g/10~6 cells/d over the 7-d culture.