Feasibility of Dermal Substitute Construction on Hybrid Scaffold Made of Poly(epsilon-caprolactone) and Bio Mimetic Fibrin Composite

摘要

Non-healing wounds can be a major problem in diabetic and burn victims. Identified causes of chronic wound formation include angiopathy, neuropathy, infection and loss of extra cellular matrix (ECM) due to increased protease action. Currently available conventional therapy is not efficient enough to promote wound healing. Tissue-engineered skin substitutes are now considered a better strategy for chronic wound management. Non-toxic scaffolds on which cells grow and replace the lost ECM are important components for tissue engineering. Degradable polymers are preferred because they may be absorbed by the time the tissue regenerate. We attempted to fabricate a degradable membrane-like and porous poly(epsilon-caprolactone)(PCL) scaffold to favour in-growth of cells, penetration of nutrients and oxygen. Modifying porous PCL membrane using a fibrin-based bionnimetic matrix could promote cell attachment and growth of tissue. The benefit of such a hybrid scaffold for long-term cell growth and ECM deposition, parallel to the degradation of polymer and diminution of mechanical property, was evaluated. We concluded that the hybrid scaffold we developed is suitable for dermal tissue construction in vitro. When grown on the hybrid scaffold, cells synthesized and deposited insoluble ECM proteins, thus proposing better epithelialization and angiogenesis when such dermal tissue constructs are implanted for treating non-healing chronic wounds.