Erratum: Signal transduction mechanism leading to enhanced proliferation of primary cultured adult rat hepatocytes treated with royal jelly 57-kDa protein (Journal of Biochemistry (2002) 132:6 (911-919))

摘要

Purification and Deglycosylation of the 57-kDa Protein—The 57-kDa protein purified by column chromatography showed a single band on native PAGE and SDS—PAGE as we have described previously (28). The band of the native 57-kDa protein shifted to a position equivalent to 48 kDa after enzyme treatment. The 57-kDa protein was stained by the periodic acid Schiff reagent, but the deglycosylated 57-kDa protein was not (Fig. 1). These results indicate that the 57-kDa protein is a glycoprotein and that the oligosaccharide chains were removed by iV-glycosidase F.