Antigenic characteristics of fowl adenovirus-4 (FAV-4) isolates from inclusion body hepatitis-hydropericardium syndrome of chickens

摘要

Two-dimensional (crossed) immunoeletrophoresis of 10 FAV-4 isolates revealed 6 immunoprecipitate peaks with FAV-4 antiserum. Purified isolates and standard FAV-4 strain revealed 8 structural polypeptides in SDS-PAGE ranging between 18 to 116 KD in molecular weight. Out of the eight polypeptides, only six polypeptides between 35-166 KD molecular weight were found to be immunogenic in immunoblotting study. This indicated no antigenic differences in the FAV-4 isolates from IBH-HPS cases.