Immobilization of a cyclodextrin glucanotransferase (CGTase) onto polyethylene film with a carboxylic acid group and production of cyclodextrins from corn starch using CGTase-immobilized PE film

摘要

Carboxylic acid groups were introduced onto polyethylene (PE) film by radiation-induced graft copolymerization. Subsequently, the clodextrin glucanotransferase (CGTase) was immobilized on the PE film with a carboxylic acid group. The activity of the immobilized CGTase on PE film was in the range of 0.40-1.04 U/cm(2) per min. The production of cyclodextrins (CDs) from corn starch was examined using the CGTase-immobilized PE film. The production ratios of CDs using CGTase-immobilized PE film was in the following order: alpha-CD > beta-CD > gamma-CD. (C) 2002 Wiley Periodicals, Inc. [References: 21]