Response of multiple generations of beet armyworm, Spodoptera exigua (Hcobner), feeding on transgenic Bt cotton

摘要

Development, reproduction and food utilization of three successive generations of beet armyworm, Spodoptera exigua (Hcobner), fed on transgenic and non-transgenic Bt cotton were examined. Significantly longer larval life-span and lower pupal weight were observed in three successive generations of S. exigua fed on transgenic Bt cotton compared with non-transgenic Bt cotton. Significantly higher survival rate and adult fecundity of S. exigua were found in three successive generations of S. exigua fed on transgenic Bt cotton compared with non-transgenic Bt cotton. The survival rate and adult fecundity of S. exigua were occurred significant increase in the third generation compared with the first generation after feeding on transgenic Bt cotton. Significantly lower consumption, frass and relative growth rate (RGR) were observed in three successive generations of S. exigua fed on transgenic Bt cotton compared with non-transgenic Bt cotton. Cotton variety significantly affected all indices of larval consumption and utilization in three successive generations of S. exigua, except for efficiency of conversion of ingested food. However, beet armyworm generation only significantly affected RGR of S. exigua. The results of this study indicated food quality on the diet-utilization efficiency of S. exigua was different along with beet armyworm generation. Measuring multigenerational development and food utilization of S. exigua at individual and population level in response to Bacillus thuringiensis (Bt) can provide a more meaningful evaluation of long-term population dynamics than experiments on a single generation. It is imperative to develop an appropriate multigenerational pest management tactic to monitor the field population dynamics of non-target pests (e.g., beet armyworm) in agricultural Bt cotton ecosystem.