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EFFECTS OF LEAF AGE ON EFFICACY OF BOLLGARD IIAGAINST BEET ARMYWORM, SPODOPTERA EXIGUA

机译:叶绿血对棉铃虫Iiagainst甜菜菌,Spodoptera exigua疗效的影响

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Three cotton varieties were grown in the field and bioassayed in the laboratory against the beet armyworm (BAW), Spodopteraexigua. A non-B.t. standard, DPL 5415, was compared to NuCotton 33B, which produces the Cry1Ac protein, and NuCotton33BII, which produces both the Cry1Ac and Cry2Ab proteins. Four leaf ages (3rd, 6th, 9th and 12th leaf from the terminal) wereused in the bioassay to evaluate effects of leaf age. Bioassays were initiated with one day old larvae and run for 10 days or 5 dayold larvae and run for 8 days. The bioassays generally showed increased mortality and decreased weight of surviving larvae withincreased leaf age for all three varieties. NuCotton 33BII showed excellent activity against BAW. The greatest survival inNuCotton 33BII occurred on the younger leaves, but surviving larvae had grown little or none and were generally about 1/10the weight of larvae on DPL 5415. It is extremely unlikely that any of the larvae on NuCotton 33BII would have been able todevelop to the adult stage.
机译:在田野中生长了三个棉花品种,并在实验室中生物测量,对抗甜菜羊虫(BAW),Spodopteraexigua。一个非B.T。标准DPL 5415与Nucotton 33b进行比较,其产生Cry1Ac蛋白和Nucotton33BII,其产生Cry1Ac和Cry2ab蛋白。在生物测定中,四个叶片(末端3,第6,第9和第12叶,评估叶时代的效果。用一天大的幼虫开始生物测定,并在10天或5天幼虫,运行8天。生物测定通常表现出增加的死亡率,并且在所有三个品种中征生叶寿中的存活幼虫重量增加。 Nucotton 33BII对BAW表现出优异的活动。最伟大的生存innucotton 33bii发生在较年轻的叶子上,但存活的幼虫已经生长很少或没有,并且通常在DPL 5415上的幼虫重量约为1/10。Nucotton 33bii的任何幼虫都是能够的到成年阶段。

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