Optimization of site-directed mutagenesis. 1. New random-centroid optimization program for Windows useful in research and development

摘要

A program of random-centroid optimization was written for Windows 95. The advantages of this new program, over the previous program written in Quick Basic, are easier utilization using a comprehensive step-by-step menu and more efficient and objective mapping. The new program markedly improved the optimization efficiency, as applied to five multimodal functions. Global optimums were found for all functions after 30-40 experiments. Even for the most difficult model, optimization was achieved in similar to 50 experiments. A graphical approach enabled by programming in the Windows environment can be a powerful tool for solving nonlinear, multimodal optimization problems, which may be encountered in research and development in chemistry and biology. [References: 22]