The Role of Intracellular Calcium Signals in Inflammatory Responses of Polarised Cystic Fibrosis Human Airway Epithelia

摘要

Hyperinflammatory host responses to bacterial infection have been postulated to be a key step in the pathogenesis of cystic fibrosis (CF) lung disease.Previous studies have indicated that the CF airway epithelium itself contributes to the hyperinflammation of CF airways via an excessive inflammatory response to bacterial infection.However,it has been controversial whether the hyperinflammation of CF epithelia results from mutations in the CF transmembrane conductance regulator (CFTR) and/or is a consequence of persistent airways infection.Recent studies have demonstrated that intracellular calcium (Ca~(2+)i) signals consequent to activation of apical G protein-coupled receptors (GPCRs) by pro-inflammatory mediators are increased in CF airway epithelia.Because of the relationship between Ca~(2+)i mobilisation and inflammatory responses,the mechanism for the increased Ca~(2+)i signals in CF was investigated and found to result from endoplasmic reticulum (ER) Ca~(2+) store expansion.The ER Ca~(2+) store expansion imparts a hyperinflammatory phenotype to chronically infected airway epithelia as a result of the larger Ca~(2+)i mobilisation coupled to an excessive inflammatory response following GPCR activation.The ER expansion is not dependent on ER retention of misfolded AF508 CFTR,but reflects an epithelial response acquired following persistent luminal airway infection.With respect to the mechanism of ER expansion in CF,the current view is that chronic airway epithelial infection triggers an unfolded protein response as a result of the increased flux of newly synthesised inflammatory mediators and defensive factors into the ER compartment.This unfolded protein response is coupled to X-box binding protein 1 (XBP-1) mRNA splicing and transcription of genes associated with the expansion of the protein-folding capacity of the ER (e.g.increases in ER chaperones and ER membranes).These studies have revealed a novel adaptive response in chronically infected airway epithelia,where the increased protein secretory capacity serves to promote epithelial homeostasis by increasing both the secretory and the reparative capacity of the cell.In addition,the increased ER-derived Ca~(2+)i signaling allows the epithelia to amplify its inflammatory responses to infectious agents and exogenous toxicants.This review is devoted to a discussion of these recent findings and their implication for Ca~(2+)i-dependent hyperinflammatory responses in CF airways.