Synthesis and properties of novel asymmetric monomethine cyanine dyes as non-covalent labels for nucleic acids

摘要

Sixteen,novel monomethine cyanine dyes with benzothiazolo-[3.2-a]-pyrimidinium,4-methyl-(5H)-pyrido-[1,2-a]-pyrimidinium,1-cyano-4-methyl-(3H)-benzothiazolo-[3,2-a]-pyridinium and 4-methyl-8-methoxy-(8H)-benzothiazolo-[3,2-a]-pyrimidinium end groups have been synthesized.Their chemical structures and purity were confirmed by ~1H NMR and elemental analysis.The longest wavelength absorption maxima of the studied dyes are in the region 450-500 nm.Most of the dyes have very high molar absorptivities,usually over 100000 lmol~(-1)cm~(-1).The investigated dyes have low fluorescence in their free form but some of them become strongly fluorescent after binding to DNA.