A 43-kDa protein from the leaves of the herb Cajanus indicus L. modulates chloroform induced hepatotoxicity in vitro.

摘要

A 43-kDa protein isolated from the leaves of the herb Cajanus indicus L. has been shown to possess a protective role against drug- and toxin- induced hepatotoxicity both in vivo and in vitro. The current study was conducted to evaluate its protective action against chloroform (CHCl3)-induced cytotoxicity in hepatocytes. Cellular viability and biochemical parameters such as glutamate pyruvate transaminase (GPT) and lactate dehydrogenase (LDH) release from the cells were measured. In addition, the antioxidant effect of the protein was investigated from the DPPH radical scavenging assay and by determining the levels of the antioxidant enzyme catalase (CAT), cellular reserves of reduced glutathione (GSH), and lipid peroxidation end products (measured as TBARS). Treatment of the cells with CHCl3 decreased cellular viability and increased GPT and LDH. Cells treated with the protein before and immediately after CHCl3 application showed a marked improvement in their viability and reduced leakage of GPT and LDH. The levels of CAT and GSH, which were diminished in cells treated with CHCl3, were restored by protein treatment. CHCl3 induced enhancement of lipid peroxidation in hepatocytes was significantly reduced by protein treatment. Results of the DPPH assay with the protein showed its radical scavenging activity. This data suggests that the protein possesses protective activity against CHCl3-induced cytotoxicity in hepatocytes and protects against CHCl3-induced hepatic damage.