Construction of pINC-lacZ Retroviral Vector and its Expression in Mouse Embryonic Stem Cells

摘要

A rctroviral vector pINC-lacZ containing an E. coli β-galactosidasc (β-gal) gene was constructed and introduced into the MESPU-13 cells by clectroporation. Four G418-rcsistant colonies were obtained from 1 X 10~7 electroporated MESPU-13 cells. Histochemical staining for β-gal activity showed that lacZ gene was expressed in at least three of the four colonies. Southern analysis proved that one copy of foreign gene was integrated in the chromosome of one of the transformed lines (MC15). These results showed that the expression of lacZ gene driven by SiCMVIE promoter can be detected in the transformed MESPU-13 cells.