Detection of enteropathogens associated with travelers' diarrhea using a multiplex Luminex-based assay performed on stool samples smeared on Whatman FTA Elute cards

摘要

We evaluated the limits of detection (LoD) for an 11-plex PCR-Luminex assay performed on Whatman (TM) FTA Elute cards smeared with stool containing pathogens associated with travelers' diarrhea. LoDs ranged from 102 to 10(5) CFU, PFU, or cysts/g for most pathogens except Cryptosporidium. Campylobacter and norovirus LoDs increased with prolonged storage of cards. (C) 2015 Elsevier Inc. All rights reserved.