In vitro rooting of quince cv. MC as rootstock for pear and acclimatization of the rooted microcuttings.

摘要

The objective of this work was to determine the auxin type and concentration to promote in vitro rooting of quince (Cydonia oblonga cv. MC) as rootstock for pear (Pyrus spp.), and to evaluate the survival of rooted microcuttings during acclimatization under ex vitro conditions. The treatments consisted of 3 auxin types (IBA, NAA and IAA) at 5 concentrations (0, 5, 10, 15 and 20 micro M). The microcuttings were cultivated for 7 days in 1/2 Murashige and Skoog's medium containing myo-inositol (100 mg litre-1), sucrose (30 g litre-1), agar (6 g litre-1) and auxin. The microcuttings were then transferred to a new medium without auxin. Rooted microcuttings originating from several treatments were acclimatized under ex vitro conditions. The auxins (IBA, NAA, and IAA) had the same effect on rooting percentage, and rooting was greatest when the auxins were applied at 10 micro M. NAA resulted in the greatest mean root length. Callus formation increased with auxin concentration, being more pronounced with IBA and NAA. Plant survival of 65.12% was obtained after 30 days of acclimatization.