首页> 外文期刊>Biochimica et Biophysica Acta. Molecular and cell biology of Lipids >Increased accumulation of the glycoxidation product Afe-(carboxymethyl)lysine in hearts of diabetic patients: generation and characterisation of a monoclonal anti-CML antibody
【24h】

Increased accumulation of the glycoxidation product Afe-(carboxymethyl)lysine in hearts of diabetic patients: generation and characterisation of a monoclonal anti-CML antibody

机译:糖尿病患者心脏中糖氧化产物Afe-(羧甲基)赖氨酸的积累增加:单克隆抗CML抗体的产生和表征

获取原文
获取原文并翻译 | 示例

摘要

Heart failure is a condition closely linked to diabetes. Hypergiycaemia amplifies the generation of a major advanced glyeation end product N~ε-(carboxymethyl)lysine (CML), which has been associated with the development of vascular and inflammatory complications. An increased accumulation of CML in hearts of diabetic patients may be one of the mechanisms related to the high risk of heart failure. Therefore, we investigated the localization of CML in diabetic hearts. To investigate the presence and accumulation of CML in tissues, a monoclonal anti-CML antibody was generated and characterised. With this novel monoclonal antibody against CML, the localization of CML was investigated by immunohistochemistry, in heart tissue of controls (n = 9) and heart tissue of diabetic patients (n = 8) without signs of inflammation or infarction. In addition, in the same subjects we studied the presence of CML in renal and lung tissues. CML staining was approximately sixfold higher in hearts from diabetic patients as compared to control hearts (2.0 ± 0.3 and 0.3 ± 0.2 A.U., respectively, P<0.01). CML deposition was localized in the small intramyocardial arteries in endothelial cells and smooth muscle cells, but not in cardiomyocytes. These arteries did not show morphological abnormalities. The intensity of staining between arteries at the epicardial, midcardial and endocardial side did not vary significantly within patients. In renal tissues, CML staining was most prominent in tubules and in atherosclerotic vessels, without differences in intensity between controls and diabetic patients. In non-infected lungs, no CML was detected. In conclusion, CML adducts are abundantly present in small intramyocardial arteries in the heart tissue of diabetic patients. The accumulation of CML in diabetic hearts may contribute to the increased risk of heart failure in hypergiycaemia.
机译:心力衰竭是与糖尿病密切相关的疾病。高血糖血症会放大主要晚期滑行终产物N〜ε-(羧甲基)赖氨酸(CML)的生成,后者与血管和炎症并发症的发展有关。糖尿病患者心脏中CML积累的增加可能是与心力衰竭高风险相关的机制之一。因此,我们调查了CML在糖尿病心脏中的定位。为了研究CML在组织中的存在和积累,产生了单克隆抗CML抗体并进行了表征。使用这种针对CML的单克隆抗体,通过免疫组织化学研究了CML在对照(n = 9)的心脏组织和糖尿病患者(n = 8)的心脏组织(无炎症或梗塞迹象)中的定位。此外,在同一主题中,我们研究了肾脏和肺组织中CML的存在。与对照心脏相比,糖尿病患者心脏的CML染色大约高六倍(分别为2.0±0.3和0.3±0.2 A.U.,P <0.01)。 CML沉积位于内皮细胞和平滑肌细胞中的心肌小动脉内,而心肌细胞内则没有。这些动脉未显示形态异常。在患者中,心外膜,心中膜和心内膜侧的动脉之间的染色强度没有显着变化。在肾组织中,CML染色在肾小管和动脉粥样硬化血管中最明显,而对照组和糖尿病患者之间的强度没有差异。在未感染的肺中,未检测到CML。总之,CML加合物大量存在于糖尿病患者心脏组织的小心肌内动脉中。糖尿病性心脏中CML的积累可能导致高血糖症的心力衰竭风险增加。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
机构文献详情
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号