Immunoadjuvant activities of E. coli- and plasmid-expressed recombinant chicken IFN-alpha/beta, IFN-gamma and IL-1beta in 1-day- and 3-week-old chickens.

摘要

The capacity of recombinant Escherichia coli- or plasmid-expressed chicken interferons (IFN) and chicken interleukin 1beta (IL-1beta), to exert immunostimulatory activities for humoral immune responses, was assessed in day-old and adult chickens. Both recombinant E. coli-expressed chicken IFN-alpha/beta and IFN-gamma facilitated the induction of a primary and also a secondary antibody response, using tetanus toxoid (TT) as a bacterial model antigen, in immunologically mature 3-week-old chickens. Incontrast, no improvement of antibody responses were noted when either type of chicken IFN was co-injected with inactivated infectious bursal disease virus (IBDV) antigen. TT-specific antibody formation was marginally increased by co-injection of recombinant E. coli-expressed chicken IL-1beta. Combined administration of IFN-alpha/beta plus IFN-gamma or IL-beta increased responses to TT in an additive, but not synergistic fashion. No augmentation of antibody responses specific for TT, nor IBDV, was notedin day-old birds, receiving IFN-alpha/beta or IFN-gamma as adjuvant. Intramuscular immunization of 3-week-old birds, using plasmids encoding IFN-alpha/beta together with TT protein antigen, significantly increased the speed and magnitude of TT-specificantibody responses. Plasmids encoding chicken IL-beta or IFN-gamma had a minimal or inhibitory effect, respectively. It is concluded that there is potential for chicken cytokines as immunoadjuvant for particular types of chicken vaccine antigens.