A universal anti-HIV-1 Tat epitope vaccine that is fully synthetic and self-adjuvanting

摘要

Circulating HIV-1 Tat protein is essential for maintenance of the chronic HIV replication that predicates both HIV transmission and clinical progression to AIDS/death. A synthetic anti-Tat epitope vaccine (TUTI-16) was designed to induce neutralizing antibodies to Tat and, hopefully, provide immunological control of HIV replication TUTI-16 is composed of (1) a conserved Tat B cell epitope (Tat 4-12), tendered universal by introducing known variant amino acids at variable positions 7.9 and 12 during solid phase synthesis, (2) a promiscuous T helper sequence and (3) a lipopeptide toll-like receptor 2 (TLR2) agonist TUTI-16 induced high titer antibodies against all 8 known variants of the Tat epitope