A glycoprotein elicitor isolated from Colletotrichum falcatum induces defense mechanisms in sugarcane leaves and suspension-cultured cells

摘要

A high molecular weight elicitor was isolated from the mycelial walls of Colletotrichum falcatum, the red rot pathogen, and partially purified by gel filtration using Sephadex G-200. The elicitor appears to be a glycoprotein and the activity of elicitor resides in the carbohydrate moiety. The partially purified elicitor induced the accumulation of phenolics and the activities of phenylalanine ammonia-lyase (PAL) and peroxidase (PO) in sugarcane leaves and suspension-cultured cells. Significant increase in the enzyme activities and phenolic content was observed I h after elicitor treatment in both cells and leaves. Phenolic content and enzyme activities consistently increased and the maximum increase was recorded 96 h after elicitor treatment. Sugarcane cells in culture responded to C falcatum elicitor in a manner similar to sugarcane leaves.