Association of ADAM33 gene polymorphism and arginase activity with susceptibility to ventilatory impairment in wood dust-exposed workers

摘要

ADAM33 represents an important gene of susceptibility for lung function impairment. This work aimed to evaluate the association between genetic polymorphism of ADAM33 at four single nucleotide polymorphisms (T1, T2, SI, and Q1) and arginase activity with respiratory functions impairment in wood workers. The study was done to compare ventilator), functions and arginase activity of 82 wood workers and 81 controls. Genotyping was determined by using the polymerase chain restriction fragment length polymorphism method. Forced expiratory volume in the first second (FEV1), forced vital capacity (FVC), and peak expiratory flow rate (PEF) of the workers were significantly reduced compared with the controls. T I single nucleotide polymorphism (SNP) was associated with obvious decline in the FEVI, FVC, and PEF in wood workers, while T2 SNP was associated with decline in FEY! and PEF. A significant increase in arginase activity was found in T2 and SI SNPs of the exposed workers. Increase in duration of exposure was correlated with the decline in ventilatory functions. This inverse correlation was significant for pulmonary function indices in AA and GG genotypes of TI and T2, respectively. Moreover, significance was detected for FVC and FEVI in AA and GA genotypes of SI and Q I. A positive correlation between arginase activity and duration of exposure was found to be significant in GG genotype of SI SNP. An association between ADAM33 gene polymorphism and impaired lung functions was detected in wood dust-exposed workers. Arginase activity may play an associated important role in increasing this impairment in wood workers.