Comparison of Lisianthus (Eustoma grandiflorum) Cultivars Based on the Selected Regeneration Media Using Anther Culture

摘要

Double haploids in lisianthus cultivars were produced using anther culture. The anther of 'Art Peach' was cultured on the Murashige and Skoog (MS) medium with 3% sucrose with auxin and cytokinin. The highest frequency of calli developed from anthers (100%) and green plantlets per100 calli (60 regenerants) was observed on the medium containing 8 mgL~(-1)N6 benzyladenine (BA) and 0.1mg-L~(-1) naphthaleneacetic acids (NAA). When anthers were cultured on the medium supplemented with 2 mg L~(-1) BA and 0.5 mg-L~(-1) NAA, 33.3% of calli formed plantlets. Using the anther culture, callus induction and plantlets regeneration in five cultivars were developed differently, suggesting that the genotype might predetermined anther culture response. These resultsindicate that a highly efficient new microprogation technique can be provided by anther culture in vitro for lisianthus. Anther culture in vitro can provide a highly efficient new microprogation technique for lisianthus.