Evaluation of Genetic Relationships among Some Persian Cultivated and a Wild Pomegranate Accessions Using RAPDs and SSRs Molecular Markers

摘要

Twenty-eight cultivated pomegranates, one hybrid seedling and one wild accession from three provinces (five eco-geographical regions) of Iran were used for the determination of their genetic relationships by using RAPD markers. Sixty-seven RAPD decamer primers were screened, out of which twenty were amplified good, reproducible and polymorphic fragments among accessions. From a total of 309 recorded fragments, 174 were polymorphic between accessions (57.64%). The Jaccard's similarity coefficients ranged from 0.15 (between the wild accession and Poost-Sefide-Torsh cv.) to 0.95 (between 'Shirin-Shahvar' and 'Shirine-Hastei'). The collective resolving power (R_p) rate value of 36.08 was scored for all RAPD primer bands. UPGMA dendrogram clustered the accessions in agreement with their geographical origins. In addition, nine SSRs loci were examined on these accessions that only one of them was polymorphic. This study revealed that the wild accession of pomegranate was genetically different from domesticated ones, indicating that wild genotypes are worthy for consideration in breeding programs of pomegranate.