Multi-Walled Carbon Nanotubes (MWCNT)-Ionic Liquid-Modified Carbon Paste Electrode: Probing Furazolidone-DNA Interactions and DNA Determination

摘要

The interactions of furazolidone (Fu) with double-stranded calf thymus DNA (dsDNA) on the multi-walled carbon nanotubes-ionic liquid-modified carbon paste electrode (MWCNT-IL-CPE) have been studied by cyclic voltammetry. In the presence of DNA, the cathodic peak current of Fu decreased and the peak potential shifted to a positive potential, indicating the intercalative interaction of Fu with DNA. The binding constant of Fu with DNA and stoichiometric coefficient has been determined according to the Hill's model. This electrochemical method was further applied to the determination of DNA. Two linear calibration curves were obtained for DNA detection in the concentration ranges of 0.03 – 0.10 and 0.10 – 4.0 μgl~(-1) with a detection limit of 0.027 μgl~(-1). The method was successfully applied to analyze Fu in serum samples.