RAPD Analysis for Genetic Variation within the Endangered Quillwort Isoetes hypsophila (Isoetaceae)

摘要

The genetic variation in the critically endangered species Isoetes hypsophila was investigated using Random Amplified Polymorphism DNA (RAPD) markers. Thirteen primers were screened from sixty primers, and a total of 104 DNA fragments were scored, ofwhich, 52 were polymorphic loci. Low-level genetic diversity within populations with PPB values ranging from 7.69% to 25.96% was found. An Analysis of Molecular Variance (AMOVA) indicated that the most of variance (78.30%) occurred between Yunnan and Sichuan, The variances among populations within regions and within populations were only 3.89% and 17.82%, respectively.