Analysis of Male Sterility-Related Proteins of Young Panicle in Rice (Oryza sativa L.) by Two-Dimensional Electrophoresis

摘要

For searching out male sterility-related proteins (polypeptides) in rice (Oryza sativa L. ), we examined the difference of panicle protein (polypeptides) between hybrid rice ( Wujin2A/R168, Wujin5A/R988) and their parents (male-sterile line Wujin2A, Wujin5A, and restorer line R168, R988) at the formation stage of pollen mother cell by two-dimensional electrophoresis (2-DE). The results revealed that the 2-DE polypeptide maps were similar among these experimental materials. A small group of polypeptides were disappeared in 2-DE polypeptide maps of male-sterile line (Wu-jin2A, WujinSA) by comparing to restorer line (R168, R988) and the first filial (F, ) generation (Wujin2A/R168, Wujin5A/R988). The isoelectric points of these polypeptides were pl 5.8-6.5, molecular weight 42. 7 X 10~3-66. 2 X 10~3.