Enantioselective Surface Plasmon Resonance Sensor Based on C60 Fullerene-Glutathione Self-Assembled Monolayer (SAM)

摘要

A novel enantioselective surface plasmon resonance (SPR) sensor based on a self-assembled monolayer of C_(60) fullerene as the chiral selector is proposed. A binding assay, apparent affinity constant, and apparent dissociation binding constant were used to analyze and study the enantioselectivity of C_(60) fullerene-glutathione film for L-histidine, which was chosen as the model analyte. The apparent affinity constant for the complex formed by L-histidine with C_(60) fullerene-glutathione film was 5.2 x 10~9M~(-1). The proposed SPR sensor can be used for the assay of L-histidine in the 10~(-10) – 10~(-7)mol/L concentration range.