Molecular cloning and sequence analysis of Zygosaccharomyces rouxii ADE2gene encoding a phosphoribosyl-aminoimidazole carboxylase

摘要

The nucleotide sequence of a 2.8 kb fragment containing the ADE2 gene of the osmotolerant yeast Zygosaccharomyces rouxii has been determined. The gene was cloned from a Z. rouxii genomic DNA library by complementation of the Saccharomyces cerevisae ade2 mutant strain. The sequenced DNA fragment contains a 1710 bp open reading frame predicting a protein of 570 amino acids. The deduced amino acid sequence shares a high degree of homology with Ade2p homologues in five other yeast species. The Gene Bank Accession No. of Z-ADE2 is AJ133372. Copyright (C) 1999 John Wiley & Sons, Ltd.