cDNA-AFLP profiling for the fiber development stage of secondary cell wall synthesis and transcriptome mapping in cotton

摘要

Cotton fiber strength is mainly determined during the secondary cell wall deposition stage when cellulose is synthesized. We obtained cDNA of 20-25 d post anthesis (DPA) fiber from 109 F_2 progeny and developed a cotton fiber transcriptome profiling via cDNA-AFLP technology using 37 different primer combinations. The F_2 population originated from an interspecific cross between Gossypium hirsutum and Gossyp,um barbadense. One hundred and thirty-eight absence/presence polymorphic transcriptderived fragments (TDFs), with sizes ranging from 100 bp to 722 bp, were screened Of these 75 (53.62%) were polymorphic between the parents of the F_2 population. Sequencing the 75 transcripts revealed that 37 of them had been reported to be cotton fiber ESTs. Nineof 75 transcript sequences were homologous to 7 cloned cotton fiber genes, encoding cysteine proteinase, vacuolar H~+-pyrophosphatase, vacuolar H~+-ATPase, catalytic subunit, arabinogalactan protein, putative receptor protein kmase PERK1, GIA/RGA-like gibberel in response modulator and cellulose synthase. Some other transcripts may represent new gene fragments in cotton fiber development. Surprisingly, 46 of the 75 transcripts were mapped to a single linkage group. The transcriptome groups and the sequenced TDFs could serve as important resources in the functional genomsc research of cotton fiber development.