Morphology and morphometry of feline bone marrow-derived mesenchymal stem cells in culture

摘要

Mesenchymal stem cells (MSC) are increasingly being proposed as a therapeutic option for treatment of a variety of different diseases in human and veterinary medicine. Stem cells have been isolated from feline bone marrow, however, very few data exist about the morphology of these cells and no data were found about the morphometry of feline bone marrow-derived MSCs (BM-MSCs). The objectives of this study were the isolation, growth evaluation, differentiation potential and characterization of feline BM-MSCs by their morphological and morphometric characteristics. In vitro differentiation assays were conducted to confirm the multipotency of feline MSC, as assessed by their ability to differentiate into three cell lineages (osteoblasts, chondrocytes, and adipocytes). To evaluate morphological and morphometric characteristics the cells are maintained in culture. Cells were observed with light microscope, with association of dyes, and they were measured at 24, 48, 72 and 120h of culture (P1 and P3). The non-parametric ANOVA test for independent samples was performed and the means were compared by Tukey's test. On average, the number of mononuclear cells obtained was 12.29 (+/- 6.05x10(6)) cells/mL of bone marrow. Morphologically, BM-MSCs were long and fusiforms, and squamous with abundant cytoplasm. In the morphometric study of the cells, it was observed a significant increase in average length of cells during the first passage. The cell lengths were 106.97 +/- 38.16 mu m and 177.91 +/- 71.61 mu m,respectively, at first and third passages (24 h). The cell widths were 30.79 +/- 16.75 mu m and 40.18 +/- 20.46 mu m, respectively, at first and third passages (24 h). The nucleus length of the feline BM-MSCs at P1 increased from 16.28 mu m (24h) to 21.29 mu m (120h). However, at P3, the nucleus length was 26.35 mu m (24h) and 25.22 mu m (120h). This information could be important for future application and use of feline BM-MSCs.