Qualitative detection of haptoglobin mRNA in bovine and human blood leukocytes and bovine milk somatic cells.

摘要

Local expression of haptoglobin (Hp) mRNA in tissue homogenates from different sites of the bovine mammary gland has recently been established. Besides alveolar and stromal tissue, blood cell is another potential cellular location of Hp expression. This study was conducted to investigate whether bovine leukocytes might contribute to the Hp mRNA transcripts found in the mammary glands after their migration into this organ. RT-PCR was carried out with RNA extracted from leukocytes in blood and from somatic cells in milk of 3 dairy cows. For comparison, human leukocytes of 4 donors were also examined. Both bovine blood and milk somatic cells showed distinct signals for Hp mRNA in all samples in contrast to human leukocytes, where Hp mRNA was detected in only 2 out of 4 donors after reamplification. It was concluded that the presence of Hp mRNA in blood-derived leukocytes indicated that these cells contributed to the Hp mRNA transcripts found in the bovine mammary glands. However, it should be clarified whether other cells within the mammary glands were also capable of Hp mRNA synthesis..