Characterisation of Pasteurella multocida isolated from rabbits in the Czech Republic

摘要

Twenty seven Pasteurella multocida rabbit isolates were characterised by pulsed-field gel electro-phoresis (PFGE) generated by restriction endonuclease Apal, and examined for the presence'of capsule and fim-briae, and tested of haemolytic activity andendotoxin release in presence of rabbit plasma. Among the isolates a high number (n = 20) of different PFGE types was observed. All isolates were found to be encapsulated and four of them also possessed fimbriae. Although the presence of the aphA gene,conferring a haemolytic phenotype for Escherichia coli under anaerobic conditions, was indicated in all of the isolates by PCR, clear haemolytic activity was observed in only one isolate when grown anaerobically. All of the isolates grew easily in the presence of rabbit plasma which showed a high capacity to bind the released endotoxin.