Authentication of Hybrid Purity in Eggplant (Solanum melongena L) Through RAPD Analysis

摘要

For testing seed purity and trueness, GOT (Grow Out Test) is a long term process and therefore, present study was carried out during Rabi-2011. The experimental materials used in the study comprised of four parents viz., Green Long (GL), Pusa Purple long (PPL), IBWL-2007-1 (IBWL), Punjab Sadabahar (PS) and their six Fi's and standard check PH-6. RAPD marker was used to test the hybridity. Out of twenty-nine, only four primers showed polymorphism, namely OPA -14, OPA-08, OPH-01 and OPH-20. According to banding pattern for the primer OPH-01, hybrid between P4and P2 (IBWL-2007-1 X Green Long) resembles to male parent i.e.. Green Long (P2) and in same reciprocal cross Pzand P4 (Green Long X IBWL-2007-1), the banding patterns of Fi hybrid resembles maleparent i.e. IBWL-2007-1 (P4). In another hybrid, between Paand P4 (Punjab Sadabahar X IBWL-2007-1) banding pattern of Fi hybrid resembles to male parent i.e., IBWL-2007-1 (P4). For the primer OPH-20, Fi hybrid between Pi and P3 (Punjab Sadabahar X IBWL-2007-1) banding pattern of Fi hybrid resembles to male parent ie. IBWL-2007-1 (Ps).For the primer OPA-14, Fi hybrid between Paand P4 (IBWL-2007-1 X Pusa Purple Long) banding pattern of Fi hybrid resembles to male parent i.e. Pusa Purple Long (P4)and in the same reciprocal cross P4and P3 (Pusa Purple Long X IBWL-2007-1) banding pattern of Fi hybrid resembles to male parent i.e. IBWL-2007-1 (P3). For primer OPA-18, in Fi hybrid between Pi and P3 (Punjab Sadabahar X IBWL-2(K)7-1), the banding pattern resembles to male parent i.e. IBWL-2007-1 (P3).