STRUCTURAL STUDY AND SEROLOGICAL CHARACTERISATION OF THE O-SPECIFIC POLYSACCHARIDE OF HAFNIA ALVEI PCM 1185, ANOTHER HAFNIA O-ANTIGEN THAT CONTAINS 3,6-DIDEOXY-3-[(R)-3-HYDROXYBUTYRAMIDO]-D-GLUCOSE

摘要

The O-specific polysaccharide of H. alvei strain PCM 1185 contains D-glucose, D-glucuronic acid, 2-acetamido-2-deoxy-D-glucose, and 3,6-dideoxy-3-[(R)-3-hydroxybutyramido]-D-glucose (Qui3NAcyl) in the ratios 2:1:1:1, as well as O-acetyl groups. On the basis of sugar and methylation analyses of the polysaccharide before and after chemical modifications (O-deacetylation, carboxyl reduction, Smith degradation), as well as H-1 and C-13 NMR spectroscopy, including 1D sequential, selective spin-decoupling, 2D homonuclear, and C-13, H-1 heteronuclear correlation spectroscopy (COSY), and 2D rotating-frame NOE spectroscopy, it was found that the polysaccharide has a pentasaccharide repeating unit with the following structure: [GRAPHICS] with O-acetyl groups present in nonstoichiometric amounts, mainly at position 2 of GlcA and position 6 of GlcNAc or lateral Glc. Serological study showed that H. alvei strain PCM 1185 can be placed in a new serotype D and that an O-acetyl group can be a part of its epitope. (C) 1996 Elsevier Science Ltd. [References: 33]