Pharmacokinetics of 20(S)-25-methoxyl-dammarane-3beta, 12beta, 20-triol and its active metabolite after oral and intravenous administration in rat.

摘要

The pharmacokinetics behaviour of 20(S)-25-methoxyl-dammarane-3beta, 12beta, 20-triol (25-OCH3-PPD) and its active metabolite after oral and intravenous administration in rats were studied. Rats were administered 2.5, 5.0, and 10 mg kg(-1) 25-OCH3-PPD orally after an overnight fasting or by intravenous injection of 5 mg kg(-1) 25-OCH3-PPD via the tail vein. Plasma concentration-time profiles of 25-OCH3-PPD and its active metabolite 25-O-demethylated (25-OH-PPD) in rats were monitored by liquid chromatography-tandem mass spectroscopy (HPLC-MS-MS). The 25-O-demethylated metabolite appears to be a pathway in the phase I metabolism of 25-OCH3-PPD in rats. The plasma concentration of the metabolite was higher than that of the parent compound.