Investigation on DNA binding and photo-cleavage properties of water-soluble porphyrin and metalloporphyrins

摘要

A water-soluble porphyrin,tetrakis[4-(trimethylammonium)phenyl]porphyrin (TAPP),and its metal complexes with Zn(II),(ZnTAPP),have been synthesized and characterized by elemental analyses and u.v.-vis.spectra.The binding of the two complexes with calf thymus DNA has been investigated by absorption spectra,luminescence titrations,and viscosity measurements.The changes of the u.v.-vis.absorption spectra during the titration of these porphyrins with calf thymus DNA revealed a large bathochromic shift (upto 8-12 nm) and a hypochromicity of the porphyrins soret bands.The intensity of the fluorescence spectra of these porphyrins was enhanced.The cytotoxicity of the series of porphyrins TAPP and MTAPP [M = Zn(II),Cu(II),Fe(III),Co(III),Ni(II),Mn(III)] complexes was determined for Madin-Darby Canine Kindney (MDCK) cells and Vero cells with the method of cell culture,and were evaluated as anti-virus activity for influenza virus type A (H3N2),B and Herpes simplex virus type-1 (HSV-1,strain SM44),type-2 (HSV-2,strain 333) in vitro.Among the complexes evaluated,inhibited viral cyto-pathogenicity at lower concentrations that were found cytotoxic for the MDCK cells.Differences were observed in the 50% effective doses (based on inhibition of viral cytopathogenicity) of these complexes for a number of influenza viruses type A,B,and HSV-1,HSV-2 strains.It was found that CoTAPP displayed good inhibitory action against the virus in vitro.