A novel model to characterize the electric double layer of lectins fro Cratylia mollis (Camaratu bean) and Canavalia ensiformis adsorbed on metallic surface

摘要

The seed lectin fro Cratylia mollis (Cra) is glucose/mannose specific,similaryly to Concanavalin A,Con A,a well characterized lectin fro Canavalia ensiformis.The equilibrium properties of an adsorbed alyer may be examined basedon the redox protentials of the constitutnets of this layer.The potential for Con A and Cra was obtained by potentiostatic techniques,uisng a saline solution as suport tocontrol the distribution of charges between saturated calomel electrode and platinum electrode as te working electrode,in aerated environment.These potentials are modelled using a Chi-square statistical distribution(p<0.05);greater stability for Con A was obtained with 1.0 mg/ml,at 10degC.The positive redox potential determined for Cra(+94mV) and Con A(+88mV) indicated a high sensitivity of our electrodes which will give information on the kinetic behaviour of the bological interface.