Low molecular weight compounds from Zoanthus sociatus impair insulin secretion via Ca+2 influx blockade and cause glucose intolerance in vivo.

摘要

Cnidarians comprise a taxon with a high biodiversity of cytolitic, neurotoxic and cardiotoxic compounds, which have not been studied on insulin release. We tested the effect of a crude extract of Zoanthus sociatus (Ellis, 1767) and the low molecular weight fraction of this extract on insulin secretion in isolated rat beta -cells and also in a glucose tolerance test in vivo. We observed that the extract inhibited insulin release by reducing the amount secreted by individual beta -cells and also by silencing a fraction of the secreting population. This effect coincided with a diminished rise of intracellular Ca+2 in response to high glucose and high K+-induced depolarization. Moreover intraperitoneal administration of the low molecular weight fraction produced glucose intolerance in adult rats. The active fraction exhibited molecular weights similar to the neurotoxins described in the phylum. Our results broaden the toxic effects of cnidarian venoms and show evidence of potential modulators of voltage-gated Ca+2 channels in this group.